IMPROVED SOMATIC EMBRYOGENESIS USING L-ASPARAGINE IN WHEAT (Triticum aestivum L.)
نویسندگان
چکیده
Somatic embryos and plants were produced from mature and immature embryo derived callus of wheat. Immature embryo showed a better capacity for somatic embryogenesis and plant regeneration. Presence of 2, 4-D was shown to be essential for induction and maintenance of somatic embryogenesis. Organic nitrogenous additives (L-proline, L-asparagine and casein hydrolysate) marked an effect on somatic embryo formation when they were used in the callus maintenance medium. Histological analysis confirmed the formation of somatic embryo. The regenerated plants had the same morphology as the original plants.
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